Benesi-Hildebrand-relationship

Upon addition of the amine (AA) to the aqueous solution containing 1 and PLP, the extent of the Schiff-base (SB) formation was monitored by electronic absorption spectroscopy. A typical example of the spectral change is shown in Figure 1. Clear isosbestic points were observed for all the measurements. The apparent SB formation constants ( sb) defined by equation (1) were evaluated according to the Benesi-Hildebrand relationship in a manner that has been reported elsewhere [6]. The Ksb values in the absence of the octopus cyclophane were also evaluated. The... 

A plot of the left-hand sides against 1 /[i)]o (Benesi-Hildebrand equation) or against [D]o (Scott equation) yields equilibrium constants and molar linear absorption coefficients from the slope and ordinate intercepts of the straight lines. The equilibrium constants obey the van t Hoff relationship as long as only one CT complex occurs. 

Benesi and Hildebrand [71] derived a relationship for EDA complexes in solution as follows ... 

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