Bence-Jones protein electrophoretic mobilities

Fio. 12. Experimental reproduction of abnormal electrophoretic patterns encountered in myeloma serum. (I) serum of normal subject. Upon adding myeloma urine containing Bence-Jones protein with mobility 2.8 to this normal serum, II was obtained addition of Bence-Jones protein with mobility 1.8 gave III addition of Bence-Jones protein with mobility 2.0 gave IV. From Gutman et al. (120). 

In 1943 Moore and associates (251) reported sedimentation constants for Bence-Jones proteins obtained from the urine of four cases of multiple myeloma, S20 = 2.8 S, 3.4 S, 3.4 S, and 3.4 S, respectively. The sedimentation constant of the electrophoretically separated main abnormal component of the serum proteins was also determined in six cases of multiple myeloma. In cases 1 and 39 of Table VII (page 197), the chief components in both instances were 7-globulins with Sao = 7.1 S. In cases 40 and 42 the chief components with electrophoretic mobilities between /5- and 7-globulins had sedimentation constants S20 = 6.5 S and 7.0 S, respectively. These four abnormal serum proteins clearly were not Bence-Jones proteins. The abnormal component in case 42 was studied further by Shapiro, Ross, and Moore (319), who found it to be a very viscous protein, molecular weight about 160,000. In cases 11 and 41 (the chief component of the first with an electrophoretic mobility between 3- and 7-globulins and of the second corresponding with 3-globulins) sedimentation constants of 820 = 4.0 S and 3.0 S, respectively, were... 

Figure 1 shows diagrammatically the arrangement of the proteins of the serum in accordance with their mobilities, as would be found in a classical electrophoretical pattern obtained by electrophoresis of normal serum at pH 8.6 in veronal buffer. The Bence-Jones and myeloma globulins are also placed in areas where they were found in respect to the serum proteins indicated (see Table V for other data on these proteins). In each system, in both the hepatic and extrahepatic proteins (26), the ala-thr ratios change in the same direction as the... 

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