BaTi 2, preparation

To improve the separation of the derivatives of fatty acids with the same effective carbon number, e.g., palmitoleic (C16 1), linoleic (18 2), andmyristic (C14 0), Baty et al. (33) reported the preparation of the anthrylmethyl esters derivatives of several fatty acids (with 9-hydroxy-methylanthracene and the catalyst 2-bromo-l-methylpyridinium iodide (BMPI)) with a view to analysis by HPLC and LC-MS (with gradient elution on a ZORBAK 5-/zm Cl8 column) (see Chemical Structure 1). The excess reagents were evaporated under nitrogen at 50°C, and the de-rivatized acids were taken up in 1 ml of mobile phase prior to chromatography. This method did not allow the resolution of the C16 1, 08 2, and C14 0 esters, although HPLC data obtained for the other acids correlated well with that obtained by capillary gas-liquid chromatography. 

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