BAPTA

CAIndexName Glycine, A,A -[l,2-ethanediylbis(oxy-2,1 -phenylene)]bis [A-(carboxymetbyl)-Other Names l,2-Bis(2-aminopbenoxy)etbane-AA, lV, A -tetraacetic acid l,2-Bis(o-aminopbenoxy)eibane-NA, A, A -tetraacetic acid Etbylenedioxybis(o-pbenylenenitri-lo)tetraacetic acid 2,2 -(Etbylenedioxy)dianiline-AA, //, A -tetraacetic acid BAPTA 

Merck Index Number 957 Chemical/Dye Class Aromatic Molecular Formula C22H24N2O10 Molecular Weight 476.43 

Physical Form Beige powder Solubility Soluble in dimethyl sulfoxide Melting Point 149-159°C 

Absorption (Amax) 209 nm, 254 nm, 203 nm, 274 nm Emission (Amax) 363 nm Synthesis Synthetic methods  

Staining Applications Calcium ions zinc ions bone proteins  

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Many of these intracellular events are critical to the prohferative effects of CXCL12. For example, the CXCL12-induced effect on proliferation is dependent on calcium. Pre-treatment of pituitary adenoma cells with BAPTA-AM abohshes the CXCL12-induced increase in prohferation (Florio et al. 2006). The increase in proliferation also requires activation of Erk 1/2, as pre-treatment with PD98059, a MEK inhibitor, blocks the proliferative effect of CXCL12, and this is correlated with a decrease in Erk 1/2 phosphorylation. Similarly, the proliferative effects of... 

ADCC ADP AM ara-C ATP BAPTA antibody-dependent cellular cytotoxicity adenosine diphosphate acetoxymethyl ester cytosine arabinoside adenosine triphosphate bis- (o-aminophenoxy)-ethane-N,N,N, N -tetraacetic acid... 

Most of the fluorescent calcium indicators and their cell-permeant acetoxymethyl (AM) esters are variations of the nonfluorescent calcium chelator BAPTA and have been proposed by Tsien/134-1365 Among them Fura-2 and Indo-1 (Figure 5.22) are particularly used formeasuring Ca2+in single cells by imaging or flow cytometry/65... 

Fig. 11. Changes In gated NMR spectra during the cardiac cycle. Top panel isovolumic left ventricular pressure In a ferret heart paced at 0.99 Hz in 8 mM [Ca +]. NMR spectra were acquired at the two times indicated on the pressure record (a) 10 ms prior to stimulation (b) 75 ms after stimulation. Middle panel shows gated F NMR spectra (each from 800 acquisitions) recorded at (a) and (b), as indicated. The bound (B) and free (F) peaks of 5F-BAPTA exhibit distinct chemical shifts at approximately 8 and 2 ppm, respectively, downfield from a standard of 1 mM 6-Ftryptophan at 0 ppm. It appears that the free [Ca +] varied during the cardiac cycle. Bottom panel shows gated P spectra (400 scans) acquired at times a and b in the same heart. The major peaks correspond to phosphocreatine (0 ppm), ATP (the three peaks upfield from phosphocreatine), and inorganic phosphate (the small peak at 4-5 ppm) (Reproduced from Marban et al. Circ. Res. 1988 63 673-678 [311] with permission of Lippincott, Williams Wilkins).

Fluo-Zin1 BTC-6N Calcein Lucifer Yellow Phen Green Newport Green OG BAPTA-5N FIUO-5N... 

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