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Baculovirus, recombinant transfer vectors

The protocols described in this article will allow the reader to prepare recombinant baculoviruses for foreign gene expression in insect cells, starting with purified virus DNA and the appropriate recombinant transfer vector. [Pg.153]

Sf9 cell culture at 1-2 x 10 cells/ml 1 pig linearized BaculoGold DNA (BD Blosclences) Recombinant Baculovirus transfer vector containing the Insert... [Pg.12]

Mix 0.5 xg linearized BaculoGold DNA with 2-5 xg recombinant Baculovirus transfer vector containing the insert and allow the mixture to sit at room temperature for 5 min. [Pg.12]

Alternative bnearized AcMNPV-baculovirus DNA for the generation of recombinant baculoviruses using the transfer vector set pVLl 392/1393 are the BacPAK6 (Takara Clontech) and the Bac-N-Blue DNA (Invitrogen, Thermo Fisher Scientific). [Pg.97]

The methods described below outline (1) the construction of a representative transfer vector in E. coli, (2) co-transfection of insect cells with this transfer vector and bacmid, (3) propagation of recombinant baculovirus, (4) induction of protein expression, (5) the extraction of the protein from insect cells, (6) the printing of a protein microarray, (7) the assay of a protein microarray for protein kinase activity, and (8) the assay of a protein microarray for cytochrome P450 turnover activity. [Pg.137]

See other pages where Baculovirus, recombinant transfer vectors is mentioned: [Pg.150]    [Pg.234]    [Pg.41]    [Pg.593]    [Pg.10]    [Pg.10]    [Pg.23]    [Pg.24]    [Pg.240]    [Pg.241]    [Pg.9]    [Pg.9]    [Pg.168]    [Pg.296]    [Pg.95]    [Pg.188]    [Pg.189]    [Pg.191]    [Pg.238]    [Pg.335]    [Pg.335]    [Pg.619]   
See also in sourсe #XX -- [ Pg.295 , Pg.296 ]



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Baculovirus

Baculovirus transfer vector

Baculovirus vectors

Baculoviruses

Recombinant vectors

Transfer vector

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