Bacteriophage Amplification of Bacteria

Microspot MALDI probe and overlay with matrix 

Lawn of bacteria with no virus added Lawn of bacteria with a low titer of bacteriophage added to 

8856Da. The spectrum of the MP-SS1 amplified mixture shows these same peaks indicating the presence of the Salmonella bacterium. In other experiments MP-SS1 provided no amplification of the Shigella spp., thus demonstrating the specificity of the MP-SS1 toward a Salmonella host cell. 

A majority of the work done in our laboratory has been conducted on E. coli and B. anthracis using MS2 and gamma-phage, respectively. Amplification results have been obtained from several other host bacteria and bacteriophages. Table 14.1 summarizes the materials studied. In all cases studied, no two bacteriophages contained the same proteins. 

TABLE 14.1 Summary of Host Bacteria and Phages Studied at CSM 

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