Bacteria, RFLP

Horz, H. P., Rotthauwe, J. H., Lukow, T., and Liesack, W. (2000). Identification of major subgroups of ammonia-oxidizing bacteria in environmental samples by T-RFLP analysis of amoA PCR products. J. Microbiol. Meth. 39, 197—204. 

Romano, P. and Suzzi, G. 1993. Sulphur dioxide and wine microorganisms. In Wine Microbiology and Biotechnology (G.H. Fleet, ed.), pp. 373-393. Harwood Academic Publishers, Switzerland. Ruiz, A., Poblet, M., Mas, A., and Guillamon, J.M. 2000. Identification of acetic acid bacteria by RFLP of PCR-amplified 16s rDNA and 16S-23S rDNA intergenic spacer. Ini. J. Syst. Microbiol. 50, 1981-1987. 

T-RFLP has been used to identify bacteria hving in consortia. An example of this is the identification of an aerobic microbial consortium that reductively dechlorinates trichloroethene completely to ethane [82]. T-RFLP patterns suggested that the consortium was dominated by populations belonging to three phylogenetic groups Dehalococcoid.es, Desulfovibrio and members of the Clostridiaceae. This composition was further confirmed by cloning. 

Marty, E., Buchs, J., Eugster-Meier, E., Lacroix, C., MeUe, L. (2012). Identification of staphylococci and dominant lactic acid bacteria in spontaneously fermented Swiss meat products using PCR-RFLP. Food Microbiology, 29, 157-166. 

Juvonen, R., Koivula, T., Haikara, A. (2008). Group-specific PCR-RFLP and real-time PCR methods for detection and tentative discrimination of strictly anaerobic beer-spoilage bacteria of the class Clostridia. International Journal of Food Microbiology, 125, 162-169. http //dx.doi.Org/10.1016/j.ijfoodmicro.2008.03.042. 

Stralis-Pavese N., Bodrossy L., Reichenauer TG, Weilharter A., and Sessitsch A. 2006. 16S rRNA based T-RFLP analysis of methane oxidising bacteria-Assessment, critical evaluation of methodology performance and application for landfill site cover soils. Appl. Soil Ecol. 31, 251. 

---