Bacitracin composition

Bacitracin has little or no optical activity (43). It is particularly resistant to acid hydrolysis, i.e., after 18 hours in 6 AT HCl, some peptide still remains (43,79). However, its approximate amino acid analysis (43,143) shows the following for each 100 g. of bacitracin, 11 g. of phenylalanine, 9 g. of leucine, 22 g. of isoleucine, 10 g. of glutamic acid, 17 g. of aspartic acid, 9 g. of lysine, 10 g. of histidine, 14 g. of cystine, and 1.5 g. of ammonia. Perhaps bacitracin also contains ornithine or hydroxylysine. The comparison of the bacitracin composition with that of subtilin is of interest, since both are products of B. svbtilis. 

Together with the derivatives of pyridine, the thiazoles soon constituted an important part of heterocyclic chemistry, as much from the point of view of the initial research as from the practical aspect. Their biological and pharmaceutical interest is in fact important as they appear in the composition of certain vitamins such as vitamin Bj (thiamine) and in the penicillins. Reduced thiazoles serve in the study of polypeptides and proteins and occur as structural units in compounds of biological significance, for example, firefly luciferins and in antibiotics bacitracin A and... 

Preliminary experiments indicated that the activity of bacitracin production by immobilized whole cells was remarkably reduced at the second reaction when a fermentation medium was used. Furthermore, the growth of leaked bacteria was observed when a fermentation medium (starch-bouillon) was employed for bacitracin production. Therefore, the effect of medium composition on bacitracin productivity was examined. As shown in Table III, bacitracin productivity in a medium... 

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