Azotobacter vinelandii growth

A strain of Azotobacter vinelandii was cultured in a 15 m3 stirred fermenter for the production of alginate. Under current conditions the mass transfer coefficient, kLa, is 0.18 s. Oxygen solubility in the fermentation broth is approximately 8 X 10 3 kgm-3.9 The specific oxygen uptake rate is 12.5 mmol g 1 h. What is the maximum cell density in the broth If copper sulphate is accidentally added to the fermentation broth, which may reduce the oxygen uptake rate to 3 mmol g 1 h 1 and inhibit the microbial cell growth, what would be the maximum cell density in this condition ... 

Escherichia coli bacfer consists of a single type of subunit 4, 156), but P. aeruginosa bacfer consists of at least two types of subunits whose relative proportions vary with growth in a manner yet to be defined 73). Azotobacter vinelandii bacfer also has two types of subunits 60). 

Page WJ, Knosp O (1989) Hyperproduction of poly-P-hydroxybutyrate during exponential growth of Azotobacter vinelandii UWD. Appl Environ Microbiol 55 1334-1339 Park WH, Lenz RW, Goodwin S (1998a) Epoxidation of bacterial polyesters with unsaturated side chains. I. Production and epoxidation of polyesters from 10-undecenoic add. Macromolecules 31 1480-1486... 

Page, W. J. and Cornish., A. (1993). Growth of Azotobacter vinelandii UWD in fish peptone medium and simplified extraction of poly-P-hydroxybutyrate. Applied and Environmental Microbiology 59,4236-4244. 

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