Axenic culture, defined

Ftichtenbusch B, Fabritius D, Steinbiichel A (1996) Incorporation of 2-methyI-3-hydroxybutyric acid by axenic cultures in defined media. FEMS Microbiol Lett 138 153-160 Ftichtenbusch B, Fabritius D, Waltermann M, Steinbiichel A (1998) Biosynthesis of novel copolyesters containing 3-hydroxypivalic acid by Rhodococcus ruber NCIMB 40126 and related bacteria. FEMS Microbiol Lett 159 85-92... 

Ease of axenic culture on simple, completely defined, solid or liquid media [1,23]. 

For our studies, axenic cultures of Ochromonas danica were grown in a chemically defined, heterotrophic medium, as described by Aaronson (5). The green photosyn hetic cells were cultured under continuous illumination (about 5 W/m" ), at 24°C the dark-bleached strain was obtained keeping the cells in complete darkness at the same temperature. 

Axenic Literally "without strangers." A system in which all biological populations are defined, such as a pure culture. 

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