Avidin toxin conjugate

SATA has been used to form conjugates with avidin or steptavidin with excellent retention of activity (Chapter 23, Section 3.1). It also has been used in the formation of a therapeutically useful toxin conjugate with recombinant CD4 (Ghetie et al., 1990), to study syntaxin proteins (Amessou et al., 2007), to prepare bispecific antibodies (Lindorfer et al., 2001), and to make a unique polylysine conjugate as a vehicle for drug delivery (Sakharov et al., 2001). 

SATA has been used to form conjugates with avidin or steptavidin with excellent retention of activity (Chapter 13, Section 3.1). It has been used in the formation of a therapeutically useful toxin conjugate with recombinant CD4 (Ghetie etal., 1990A). 

Antibodies may be conjugated to enzymes such as alkaline phosphatase or horseradish peroxidase, or to other tags such as fluorescein or biotin (55). The fluorescein is measured by antifluorescein antibodies coupled with an enzyme, and biotin is measured by an avidin-enzyme conjugate, which binds with high affinity to biotin. Michalik et al.(47) used human albumin to tag their second antitoxin, and then used an anti-albumin antibody conjugated to peroxidase for detection of botulism toxins Aand B. They reported a sensitivity for this method of 100 and 300 MLD for toxin types A and B, respectively. 

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