Avidin sulfo-SMCC

Add 1.0 mg of sulfo-SMCC (Thermo Fisher) to each ml of (strept)avidin solution. Mix to dissolve. 

Protocol for the Conjugation of SMCC-Activated Enzymes with Thiolated (Strept)avidin Activation of Enzyme with Sulfo-SMCC... 

The following protocol describes the activation of avidin or streptavidin with sulfo-SMCC and its subsequent conjugation with an enzyme modified to contain sulfhydryls using SATA (Chapter 1, Section 4.1). A method for the opposite approach, wherein the enzyme is activated with SMCC and the avidin component is thiolated, is presented immediately after this protocol. This strategy may be the most common approach to forming these conjugates (Fig. 363). In addition, since there are enzymes commercially available that are preactivated with SMCC (Pierce), their use may be the easiest solution. 

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