Avidin reductive amination

Protocol for the Conjugation of (Strept)avidin with Ferritin Using Reductive Amination... 

Conjugation of HRP by reductive amination can be done by oxidizing the carbohydrate on the enzyme and subsequently coupling to the amines on (strept)avidin (Figure 23.5). 

Figure 23.5 Oxidation of the polysaccharide components of HRP produces reactive aldehyde groups. Conjugation to avidin then may be done by reductive amination.

Protocol for the Preparation of Avidin—HRP or Streptavidin—HRP by Reductive Amination... 

In the second case, an immobilization chemistry is required to graft avidin onto the beads. When using agarose beads, the microspheres were purchased as terminated with aldehyde groups to which proteins could be linked via reductive amination (Fig. 2B) [6]. Another possibility is to use commercially available neutravidin coated beads [7]. This affinity immobilization system enables the achievement of perfectly orientated DNA probes since the bi-... 

Fig. 2 Nucleic acid immobilization, (a) Immobilization of 5 -amino-C6 modified probes onto amino modified beads, (b) Grafting of biotinylated probes after the immobilization of avidin via reductive amination onto sepharose beads (3 xm)...

Figure 6.2 The trifunctional reagent sulfo-SBED reacts with amine-containing bait proteins via its NHS ester side chain. Subsequent interaction with a protein sample and exposure to UV light can cause crosslink formation with a second interacting protein. The biotin portion provides purification or labeling capability using avidin or streptavidin reagents. The disulfide bond on the NHS ester arm provides cleavability using disulfide reductants, which effectively transfers the biotin label to an unknown interacting protein.

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