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Autoradiography of Radioactive Labeled Compounds in Gels

Important Pay attention to the local and state rules governing the use of radioactive materials  [Pg.80]

Autoradiography is the method of choice for detection and quantification of radioactive labeled samples in electrophoresis gels, blotting, or hybridization filters using X-ray films. The main drawback of X-ray films is a limited linear range of image density with respect to the amount of radioactivity. [Pg.80]

Whereas samples containing strong radiation emitters as or are subjected to autoradiography direcdy, H, or labeled probes need an enhancement by solid-state scintillation. [Pg.80]

Mostly dried gels or membranes are used in autoradiography. If wet gels have to be exposed to films, wrap the gel into an impermeable tbin foil to prevent chemical development of the film. [Pg.80]

Sensitivity of X-ray films can be increased by use of an intensifying screen or by pre-flashing. The following protocol describes autoradiography using intensifying screen and solid scintillation, respectively. [Pg.80]


See other pages where Autoradiography of Radioactive Labeled Compounds in Gels is mentioned: [Pg.80]    [Pg.81]   


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Autoradiography

Autoradiography of gels

Compound labels

Compounds radioactive

In gels

Labelled compounds

Radioactive labelled compounds

Radioactive labelling

Radioactively-labelled

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