Autofluorescence spectral imaging

Spectral imaging can identify autofluorescence and subtract it from the spectra of the image either with spectral confocals or the spectral camera (van der Loos, 2008). 

For example, lignin fluorescence in roots, vascular tissues, and cell walls of aerial plant parts interferes with imaging at wavelengths between 490 and 620 nm while the chlorophyll autofluorescence in leaves and stems is most problematic between 630 and 770 nm. Thus imaging of GFP and its closest spectral variants such as CFP and YFP is most likely to be problematic in roots, whereas RFPs may be hard to discriminate in chloroplast containing aerial plant tissues [17], These problems have only recently been effectively... 

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