Auto-Spot Robot

ABIMED developed the Auto-Spot Robot ASP 222 to automate only the distribution of activated amino acids onto the derivatized cellulose membrane in a defined grid. Bulk operations such as deprotection and washings are still carried out manually. Predissolved solutions of Fmoc-amino acids and activator are incorporated and must be prepared immediately prior to use. The membranes contain 400 peptide spots and fit directly into the system. 

Typical membrane loadings are 0.5-0.6 jimol/cm Distribution of the 44 positions of the predissolved activated amino acids can be performed on four membranes simuitaneously. Delivery of the amino acids to the membrane is via a robotic arm system with a needle-based assembly connected to a motor-driven syringe. The ASP 222 is operated using Windows -based software on an IBM-compatible 383/484 computer to allow sequence entry manually or from external databases. 

6 ZINSSER ANALYTIC SMPS 350 multiple peptide synthesizer 

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