Atrazine conjugation with

The basis of the selectivity and differences in type of activity of the triazines has been discussed thoroughly conjugation with glutathione, via displacement of the chlorine. 

Specificity of the MAbs. At UCB we compared the spedfidly of the MAbs using the Vslassical competition EIA, which measured the ability of various triazines to compete with atrazine conjugates (immobilized on the EIA wells) for binding the MAbs. A similar set of experiments at UCD was done using the "haptenated enzyme EIA format, with simazine-N(C2)-alkaIine phosphatase as the competitor. 

Another class of immunosensors utilizes enzyme conjugates with antigens at the surface of antibody coated capillaries or thick film patterned glass plates (15). This report describes a PMT and CCD camera based immunosensor for the herbicide (2,4-D) and fertihzers (Atrazine and Simazine). The detection principle improves the sensitivity of the sensor by atleast three orders of magnitude compared to a microtitre plate assay. 

The use of capillaries to run immunoassay reactions was presented in the 1980s and 1990s by Halsall group [8] for the detection of atrazine. The system was based on a flow injection analysis protocol where different solutions (sample, conjugate) were injected and reacted in the capillary itself the affinity-captured enzyme conjugate then reacted with a substrate solution injected in the capillary prior to detection. The reacted substrate solution was then pushed towards an electrode for amperometric detection. The authors showed that the incubation time reached equilibrium after about 20 min, but they worked with a capillary of 360 pm diameter. In order to really reduce the incubation time below lmin, we developed disposable polymer chips with an internal diameter of 40 pm (almost one order of magnitude smaller than... 

The third metabolic pathway discovered for degradation of triazine herbicides in plants was first reported by Shimabukuro et al. (1970) and involved conjugation of atrazine with glutathione in corn nutrient uptake, excised leaves, and leaf disc experiments. This new degradation mechanism was postulated to be the primary factor in the... 

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