Association Zimm plot

Figure 16. Zimm plot of 514.5 nm light-scattering data characterizing associated kraft lignin sample in DMF following desalting after incubation at 170 gL 1 for 300 h in 1.0 M ionic strength aqueous 0.40 M NaOH.

Despite being an absolute method that provides very important information on the fundamental properties of the polymer in solution, including its conformation, chain stiffness and its interaction with the environment, the use of SLS is limited as it requires expensive equipment and strict experimental conditions including very pure and fully soluble polymers and dust-free. In addition, Zimm plots of experimental data can be irregularly shaped and difficult to interpret when polymer aggregation or association occurs [41]. Several examples of further studies addressing the macromolecular dimensions and conformation of chitin [42] and chitosan [40,43-48] can be found in the hterature. 

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