Assembly reagents, antibodies

Antibodies to PAMAM Dendrimers Reagents for Immune Detection, Patterning and Assembly of Dendrimers... 

BPE-phenytoin donor or phenytoin in a sample is bound by biotinylated antiphenytoin antibody. The donor is quenched by a Texas Red-avidin acceptor. The assembly is small enough to fit into a 23-gauge needle. Cycle time is from 5 to 30 min, with excellent reproducibility. The sensor has been further optimized and the reagents have been characterized by the determination of association and dissociation constants/120, 121) Intact antibody as well as Fab fragments are used, resulting in a sensor which responds well over the clinically useful concentration range of phenytoin. 

Viral structural proteins expressed by baculovirus infected insect cells assemble into multimeric structures that resemble viral core-like particles and virus-like particles (CLPs and VLPs, respectively). This presentation has brought the attention of researchers for the potential use of these structures as safe immunological reagents for virus or antibody detection in enzyme immuno assays, as vaccines, and more recently, as gene delivering systems for gene therapy [9]. 

Scheme 3. Final assembly of the immobiltation reagent incorporating dithiol tether groups and antibody immobilization functionality.

S. C. Lee et al. Antibodies to PAMAM dendrimers reagents for immune detection, patterning and assembly of dendrimers, in Dendrimers and Other Derulritic Polymers. J.M.J. Frechet and D.A. TomaUa, Eds. John WUey Sons West Sussex, pp. 559-565, 2001. 

---