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Assay of Acid Phosphatase Using Mini-Ion Exchange Columns

Assay of Acid Phosphatase Using Mini-Ion Exchange Columns [Pg.165]

Using a clean, wooden applicator stock, position a very small piece of nonabsorbent cotton in the narrow end of a 6 in. disposable Pasteur pipette, but do not pack the cotton. It is important that the cotton be [Pg.165]

Place approximately 1 ml water into each pipette. This can be done with a wash bottle. [Pg.166]

Add 1 ml of a heavy suspension of Dowex-1-formate (prepared as described in steps 4-1 to 4-8 and 4-16) to each of the columns. [Pg.166]

Wash the resin from the sides of the column with water and allow the column to settle. Do not, however, allow the column to dry out. This can be prevented by small additions of water. [Pg.166]




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Acid phosphatase

Acid phosphatase assay

Acidity exchange

Column Use

Column assay

Exchange columns

Exchange of ions

Exchangeable acidity

Exchanger column

Ion-exchange column

Mini-columns

Minis

Phosphatase assays

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