Assay of Acid Phosphatase Using Mini-Ion Exchange Columns

Assay of Acid Phosphatase Using Mini-Ion Exchange Columns 

Using a clean, wooden applicator stock, position a very small piece of nonabsorbent cotton in the narrow end of a 6 in. disposable Pasteur pipette, but do not pack the cotton. It is important that the cotton be 

Place approximately 1 ml water into each pipette. This can be done with a wash bottle. 

Add 1 ml of a heavy suspension of Dowex-1-formate (prepared as described in steps 4-1 to 4-8 and 4-16) to each of the columns. 

Wash the resin from the sides of the column with water and allow the column to settle. Do not, however, allow the column to dry out. This can be prevented by small additions of water. 

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