Assay implementation

Jordan KH, Bruner J, Doan M et al. (2000) Automated Plasma Protein Binding Assay Implementation on a Tecan Genesis 150 and and Zymark RapidPlate. Poster presented at the LRIG SouthEast October 25 2000 Judd RL and Pesce AJ (1982) Free drug concentrations are constant in serial fractions of plasma ultrafiltrates. Clin Chem 28 1726... 

Linear actuated devices control liquid movement by mechanical displacement (e.g. a plunger). Liquid control is mostly limited to a one-dimensional liquid flow (no branches or alternative paths) with the corresponding possibilities and limitations to assay implementation. The degree of integration is very high, with liquid calibrants and reaction buffers pre-stored in pouches. 

As mentioned earlier, liquid flow in microchannels is typically strictly laminar over a wide range of flow rates and channel dimensions. Pressure driven laminar flow offers several opportunities for assay implementation ... 

Chapter 5 discusses in depth the statistical considerations related to LB A development and validation. In addition to the most appropriate algorithms for describing the nonlinear calibration curves typically found in LBAs, the authors also provide further insight into the performance characteristics to be evaluated during assay validation, including the concepts of total error in prestudy validation and the use of the 4-6-X rule. The decision rules at the prestudy validation and routine assay implementation stages are also discussed in some detail in Chapter 5. 

Principally, the selection of an appropriate material for any particular type of microdevice is dependent on the microfiuidic assay implemented in the device. In practice, there is often a compromise between the desired properties of the material and the availability of a technology for micromachining or tuning the properties of the material. For this reason, in the field of microfiuidics, there has always been interest in testing and designing new materials and, at the same time, developing novel techniques for microfabrication of these materials. 

---