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In vitro Assay of L Chain Activity. Since the elucidation of the molecular mechanisms of all clostridial neurotoxins, it has become convenient to test the proteolytic activity of the L chain in vitro. For this purpose, L chain or reduced di-chain toxin is incubated with substrate protein. The cleavage products are then separated by SDS-PAGE and analyzed by immunoblotting or autoradiography. [Pg.208]

See other pages where Assay Clostridial neurotoxins is mentioned: [Pg.183] [Pg.184] [Pg.205]

See also in sourсe #XX -- [ Pg.183 , Pg.197 , Pg.204 , Pg.205 ]