Aspergillus oryzae cytochrome

We have evaluated the effect of ionic strength on the kinetic parameters of lactose hydrolysis with (3-galactosidase from Aspergillus oryzae in Mcllvaine citrate-phosphate buffer at concentrations from 50 to 400 mM and found an increase in K but almost no effect on V (kcat). Studies on the effect of ionic strength on enzyme kinetics are not usual, so that works with acetylcholinesterase (Nolte et al. 1980), myosin kinase (Blumenthal and Stull 1982) and cytochrome C (Hazzard et al. 1991 Harris et al. 1994) are worth mentioning. Combined effect of pH and ionic strength on enzyme kinetics has been recently analyzed (Alberty 2006). 

Nazir KHMNH, Ichinose H, Wariishi H (2010) Molecular characterization and isolation of cytochrome P450 genes from the filamentous fungus Aspergillus oryzae. Arch Microbiol 192 395 08... 

Cloning and enhanced expression of the cytochrome P450nor gene (nicA CYP55A5) encoding nitric oxide reductase from Aspergillus oryzae. Biosci Biotechnol Biochem 68 2040-2049... 

Fig. 3. Schematic diagram of the Arabidopsis thaliana nitrate reductase domains and the amino acid homology with nitrate reductases from other species and other proteins. Arabidopsis thaliana amino acid sequence is from Crawford et al. (1988). Other sequence data are as follows Nicotiana tabacum, Nia 2, N. sylvestris type (Vaucheret et al., 1989b) Hordeum vu/ ore(K. M. Schnorr, A. Kleinhofs, and R. L. Warner, unpublished data) Oryza sativa (Choi et al., 1989) Aspergillus nidulans (Kinghom and Campbell, 1989) rat liver sulfite oxidase (Rlso) (Crawford et al., 1988) chicken microsomal cytochrome (CM cyt b5) (Ndbrega and Ozols, 1971) cytochrome bf reductase, bovine (Ozols et al., 1985) cytochrome reductase, human (Yubisui et al., 1984).

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