Ascorbic acid oxidase molecular weight

The final stage in the biosynthesis of NA involves a second mixed function oxidase, dopamine- -hydroxylase. This enzyme has been extensively purified from the bovine adrenal medulla, and is a copper-containing protein of molecular weight 290,000. Ascorbic acid acts as the cofactor (XHj). Enzyme activity is stimulated by catalytic amounts of fumaric acid and by certain other dicarboxylic acids. The enzyme has a broad substrate specificity, and will catalyse the /7-hydroxylation of a large number of phenylethylamine derivatives, including a number of sympathomimetic amines (Table 8). 

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