Ascorbate peroxidase inactivation

Hydrogen peroxide in higher plants and algae may also be decomposed by ascorbate peroxidase. Ascorbate peroxidase inactivates hydrogen peroxide in the cytosol and chloroplasts by the following mechanism. 

Hiner AN, Rodriguez-Lopez JN, Amao MB et al (2000) Kinetic study of the inactivation of ascorbate peroxidase by hydrogen peroxide. Biochem J 348 321-328... 

Kitajima S, Shimaoka T, Kurioka M et al (2007) Irreversible crosslinking of heme to the distal tryptophan of stromal ascorbate peroxidase in response to rapid inactivation by H202. FEBS J 274 3013-3020... 

Hossain, M. A., and Asada, K., 1984, Inactivation of ascorbate peroxidase in spinach chloroplasts on dark addition of hydrogen peroxide its protection by ascorbate, Plant Cell Physiol. 25 128591295. 

Miyake, C., and Asada, K., 1996, Inactivation mechanism of ascorbate peroxidase at low concentrations of ascorbate hydrogen peroxide decomposes Compound 1 of ascorbate peroxidase. Plant Cell Physiol. 37 423n430. 

Finally, metabolism and inactivation of Cd-induced ROS by cells and tissues can also be perceived as indirect processes that contribute to Cd detoxification. The cellular antioxidative defense mechanisms responsible for ROS scavenging have been thoroughly reviewed [474,489]. They include antioxidative enzymes (e.g., superoxide dismutases, catalases, glutathione peroxidases, antioxidative metabolites (GSH, ascorbic acid, vitamin E) and the enzymes involved in their regeneration (thioredioxins, glutaredoxins, glutathione reductases). 

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