Artificial metalloenzymes chemical optimization

Fig. 2 Biotin-avidin technology Artificial metalloenzymes [M(L )(biotin-ligand)]c(strept)avidin for enantioselective catalysis are based on the anchoring of a catalyticaUy active metal fragment within a host protein via a hgand, a spacer, and biotin. Chemical optimization can be achieved either by varying the spacer or the metal chelate moiety ML ). Saturation mutagenesis at a position close to the metal moiety ( ) can be used for genetic optimization...

One of the most attractive feature of this approach is the diversity generated by combining both chemical and genetic optimization strategies. Following this approach, we have been able to develop artificial metalloenzymes for the hydrogenation, the allylic alkylation and the transfer hydrogenation reaction. 

M. Skander, C. Malan, A. Ivanova, T. R. Ward, Chemical optimization of artificial metalloenzymes based on the biotin-avidin technology (5)-Selective and solvent-tolerant hydrogenation catalysts via the introduction of chiral amino acid spacers, Chem. Commun., 2005, 4815-4817. 

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