Arthrobacter lipase catalyzed hydrolysis

Figure 1. pH Profile for Arthrobacter Lipase-catalyzed Hydrolysis of Acetate of Racemic HMPC. 

Table 11.1-16. Lipase-catalyzed enantiomer-differentiating hydrolysis of esters of racemic cyclic secondary and tertiary alcohols in aqueous solution (PFL Pseudomonasfluorescens lipase, PSL Pseudomonas sp. lipase, CCL Candida cylindracea lipase, ABL Arthrobacter sp. lipase, PCL Pseudomonas cepacia lipase, CRL Candida rugosa lipase, CE cholesterol esterase).

In a further example, a biocatalytic route for the production of optically pure 3-substituted cyclohexylamine derivatives from prochiral bicychc P-diketones was established by employing three biocatalytic reaction steps (Scheme 4.16) [53]. The sequence combined the stereoselective hydrolysis of a C-C bond catalyzed by a P-diketone hydrolase [54] (6-oxocamphor hydrolase (OCH) from Rhodococcus sp. [55]), followed by an Upase-catalyzed esterification [Candida antarctica lipase B (CAL-B), Novozyme 435], and a subsequent asymmetric amination by either an (S)-or (1 )-selective m-TA [V.fluvialis [27] or a variant of the Arthrobacter sp. TA [16a] (ArRmutll)]. 

---