Aqueous reactions dithiothreitol

Von Sonntag and coworkers14 repeated Michael and Hart s study of the reaction of OH radical with 1,3- and 1,4-cyclohexadienes and extended it. They found that in the case of 1,4-cyclohexadiene, 50% of the OH radicals abstract an hydrogen atom, while only about 25% of the OH radicals abstract an hydrogen atom from 1,3-cyclohexadiene. The remaining OH radicals probably add to the double bond. The addition to the double bond was confirmed by final products analysis in the case of the 1,4-isomer. When N20-saturated aqueous solution of 1,4-cyclohexadiene (10-2 M) together with lower (10-4 M) concentration of the thiol (1,4-dithiothreitol) was y-radiolysed, it was found that 4-hydroxycyclohexene was produced with a yield of 0.29 prnol J 1, i.e. a yield of 50% of the OH radicals (equation 9). 

Adhikari S, Sprinz H, Brede 0 (2001) Thiyl radical induced isomerization of unsaturated fatty acids determination of equilibrium constants. Res Chem Intermed 27 549-559 Adhikary A, Bothe E, Jain V, von Sonntag C (2000) Pulse radiolysis of the DNA-binding bisbenzimid-azole derivatives Hoechst 33258 and 33342 in aqueous solution. Int J Radiat Biol 76 1157-1166 Akhlaq MS, von Sonntag C (1986) Free-radical-induced elimination of H2S from dithiothreitol. A chain reaction. J Am Chem Soc 108 3542-3544... 

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Zhang N, Schuchmann H-P, von Sonntag C (1991) The reaction of the superoxide radical anion with dithiothreitol a chain process. J Phys Chem 95 4718-4722 Zhang X, Zhang N, Schuchmann H-P, von Sonntag C (1994) Pulse radiolysis of 2-mercaptoethanol in oxygenated aqueous solution. Generation and reactions of the thiylperoxyl radical. J Phys... 

Prakash Rao PJ, Bothe E, Schulte-Frohlinde D (1992) Reaction of dithiothreitol and para-nitroace-tophenone with different radical precursors of OH radical-induced strand break formation of single-stranded DNA in anoxic aqueous solution. Int J Radiat Biol 61 577-591 Prat F, Houk KN, Foote CS (1998) Effect of guanine stacking on the oxidation of 8-oxoguanine in B-DNA. J Am Chem Soc 120 845-846... 

The effect of dithiothreitol (DTT) on DNA radiolysis at cryogenic temperatures was investigated by the EPR technique in order to elucidate its properties as a radioprotector and to establish which DNA radicals can be repaired. The DTT, even at concentrations up to 1 M, does not repair more than 30% of DNA radicals. However, the ability of DTT to donate hydrogen atoms to damaged DNA in frozen aqueous solutions is limited to the allyl radical of thymine [reaction (7)] and guanine radical cation [reaction (8)] ... 

The deselenization protocol involved the treatment of Sec-containing peptides, at room temperature and in aqueous media, with excess TCEP in the presence of dithiothreitol (DTT). The utihty of the reaction was demonstrated on a model... 

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