Aquasil phases

Those possessing enhanced polar selectivity, as shown by a high hydrogenbonding capacity (i.e., a p values > 1), e.g.. Platinum CjglS EPS, Synergi Polar RP, Zorbax SB AQ, and Aquasil phases (column nos. 75, 105, 134, and 5, respectively) - group A. 

Free clopidogrel base, CCA, clopidogrel AM, and diltiazem (i.s.) Column Aquasil Ci8 column Human plasma (100 mm x 3 mm) Detector LC-MS/MS operated in electrospray ionization positive mode SRM monitoring Unchanged clopidogrel m/z 322 -> 212 Carboxyl metabolite m/z 308 -> 198 Active metabolite m/z 356 -> 212 Diltiazem 415 —> 178 Mobile phase gradient of ACN/ 0.1% formic acid... 

Biological activity was determined by the rice lamina inclination assay. HPLC support and mobile phase A, Cjg silica and 45% acetonitrile B, Cjg silica and 45% acetonitrile C, silica (Aquasil) and chloroform-methanol (95 5, 1% water). Abbreviations CS, castasterone BL, brassinolide. 

Group D Moderate surface coverage/hydrophobicity, shape selectivity, and hydrogen-bonding, and low ion-exchange capacity, as exemplified by phases of moderate retentivity and silanol activity, e.g., Nucleosil Cjg, Aquasil Cjg, Platinum Cjg, and pBondapak Cjg (column nos. 64, 5, 74, and 111, respectively). 

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