Applications to protein domains

Direct evidence for inter-domain dynamics in multi-domain proteins 

Fesik and co-workers have reported a study on the conserved two-domain protein SP14.3 from S. pneumoniae.116 Although the function of this protein was not known, it was believed to be essential for S. pneumoniae and thus represented a target for development of therapeutics. Intriguingly, although the NMR structure indicated that the C- and N-terminal domains are separated by only a three-residue linker, a single order tensor could not be determined 

An account has now appeared in which the domain-domain alignment determined using RDCs exhibits potential discrepancies with the alignment determined using NMR rotational diffusion measurements. In a study on polyubiquitin chains, Fushman and co-workers found small differences in the relative alignment of two ubiquitin units in Ub2 determined using RDCs and rotational diffusion measurements.124 The NMR study 

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