Applications of RNA Chromatography

A novel assay was developed using fuU denaturing DNA chromatography [45]. 

After treatment with UDGase when uracil is present in the DNA strand, there is a significant shift in retention time of the modified single-stranded DNA. Removal of the uracil base from the single-stranded DNA results in elution at an earlier retention time through loss of the uracil base and subsequent decrease in hydro-phobicity of the DNA. 

RNA is found in a repertoire of intra- and inter-molecular hydrogen bonding interactions. One famiUar structural element in an RNA molecule is the stem-loop, in which a noncomplementary segment separates two complementary stretches of nucleotides. 

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