Applications in Isoelectric Focusing

Isoelectric focusing has rapidly become an important tool for many researchers, mainly in biochemistry and related sciences. The two main applications of isoelectric focusing are [Pg.3]

Analytical or preparative separation of ampholytes, especially proteins, according to isoelectric point. [Pg.3]

The technique is characterized by very high analytical resolution and by simplicity of apparatus and method. Electrofocusing has been applied to many types of proteins. Proteins with as small a difference as 0.02 pH units at the isoelectric point have been successfully separated. [Pg.3]

The capabilities of measuring isoelectric points are outstanding when compared with the previously available method of free electrophoresis. [Pg.3]

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