Apparatus, materials and operating conditions

The pumping system consists of a standard Milton Roy Minipump or 

Latex samples are prepared in the eluting solution at concentrations normally in the range 0.005-0.01% by mass, and are injected through a standard multiport valve. Prefiltering (0.4 fim filter) and ultrasonication 10 s) are also recommended. 

The choice of detection device is also quite important since the resolution of size separation for a given column can be markedly affected by the signal characteristics of the detector. The most commonly employed detection is optical turbidity. As will be shown, for a given latex, the effect of detection wavelength can be critical. Thus the optimal set-up employs a variable wavelength UV-visible detector and a standard strip-chart output. 

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