Smokeless tobacco apoptosis

SMOKELESS TOBACCO AND APOPTOSIS IN HUMAN ORAL KERATINOCYTES BY FLOW CYTOMETRY AND DNA CELL CYCLE ANALYSIS... 

FIGURE 7.2 Flow cytometric analysis of cell cycle distribution and apoptosis in human oral keratinocytes in response to increasing concentrations of smokeless tobacco extract. Primary keratinocytes were grown to approximately 70% confluence and then treated with various concentrations of STE for 25h. Following incubation, the cells were removed from the culture surfaces by trypsinization, and DNA content and apoptosis were determined using the method of Telford et al. Raw data were modeled using ModFit cell cycle... 

Bagchi, M. et al.. Smokeless tobacco, oxidative stress, apoptosis, and antioxidants in human oral keratinocytes. Free Radical Biol Med., 26, 992, 1999. 

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