Apoenzyme reactivation immunoassay

Burd, J.F., Ellis, P.B., Greenquist, A.C., Li, T.M., Morris, D.L., Rupchock, P.A., Sommer, R.G., Tyhach, R.J., Walter, B. and Zipp, A.P. (1983). Measurement of theophylline with the substrate-labeled fluorescent immunoassay and apoenzyme reactivation immunoassay system in solution and in solid phase reagent strips. In Avrameas, S. et al. (Eds), Immunoenzymatic Techniques Proc. 2nd Int. Symp. Immunoenzymatic Techniques, Cannes, 16-18 March. Elsevier Science Publishers, Amsterdam, pp. 239-246. 

S107 Morikawa, A., Tajima, K., Mitsuhashi, M., Tokuyama, K., Mochizuki, H. and Kuroume, T. (1985). Simple and rapid estimation of serum theophylline concentration using apoenzyme reactivation immunoassay. Ann. Allergy 55, 72-74. 

S108 Morris, D.L. (1985). Effect of antibodies to glucose oxidase in the apoenzyme reactivation immunoassay system. Anal. Biochem. ISl, 235-241. 

SI 13 Plebani, M. and Burlina, A. (1985). Determination of serum theophylline by apoenzyme reactivation immunoassay system. Ther. Drug Monit. 7, 451-454. 

Both competitive and noncompetitive methods have been incorporated into homogeneous enzyme-labeled immunoassay kits that ultimately relate enzyme activity to analyte concentration.22 The competitive-binding assays are called enzyme-multiplied immunoassay technique (EMIT), substrate-labeled fluorescein immunoassay (SLFIA), apoenzyme reactivation immunoassay (ARIS), and cloned enzyme donor immunoassay (CEDIA), while a noncompetitive method is called enzyme inhibitory homogeneous immunoassay (EIHIA). 

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