Antimyosin immunoliposomes

The sites of targeting of IL on hypoxic cardiocytes was visualized by fluorescent microscopy, using rhoda-mine labeled antimyosin immunoliposomes. Fig. 17 shows that only cells treated with antimyosin-rhoda-mine labeled IL were still confluent in the culture and that almost all cells were labeled with fluoresent liposomes. Those cells treated with rhodamine labeled PL showed extremely sparse number of cells still attached to the culture plates at 24 h of incubation, with essentially no or minimal fluorescence. Confocal microscopic examination of the cultures treated with rhodamine labeled IL showed that the cells still retained their morphology and shape with scattered fluorescent liposomes attached to the cell membranes (Fig. 18A). Those cells treated with rhodamine labeled PL were shrunken and only a few random cells showed some non-specific attachment of fluorescent PL (Fig. 18B). In this study, untreated hypoxic cells were all dead and since there was no fluorescent compounds added in them, no micrographs were obtained. 

Khaw, B.A. Vural, I. Torchilin, V.P. Haider, N. Narula, J. Expression of antimyosin sEv gene in cardiocytes use of cytoskeleton-specific immunoliposomes for transfection. Proceedings of 23rd International Symposium on Controlled Release of Bioactive Materials Kyoto Japan, 1996, 135-136. 

Key words ATP, Liposomes, Immunoliposomes, Antimyosin, Ischemia, Isolated rat heart. Rabbit myocardial infarction... 

---