Antifade agents

Antifade agents (free-radical scavengers) in aqueous mounting media prevent photobleaching of fluorophore labels under exposure to excitation light. 

Glycerol-gel mounting medium containing an antifading agent (Vectashield Vector). 

Use the relevant visualization steps described in Subheading 3.3. for IGSS, IPO, or lAP, in sequential reactions. Sections stained for immunofluorescence should be mounted with a glycerol-gel solution containing an antifading agent such as Vectashield (Vector). 

After the final wash, the coverslips are drained of excess liquid and are mounted by inverting onto 5 pil Mowiol mountant (Cal-biochem) containing p-phenylenediamine (1 mg/ml) as an antifading agent. 

Krenik KD, Kephart GM, Offord KP, Dunnette SL, Gleich GJ (1989) Comparison of antifading agents used in immunofluorescence. J Immunol Methods 117 91-97... 

Mount the tissue under a covershp in a mounting medium containing an antifading agent see Notes 5 and 39)... 

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