Antibody-type CSPs

Poor long term stability may present one of the major obstacles for future analytical application of CSPs based on enantioselective antibodies. Hofstetter and coworkers, however, reported that in their hands anti-amino acid antibody-type CSPs, when exclusively operated with phosphate buffered saline pH 7.4, could be reused for several thousand analyses over a period of three years without any significant loss in performance [279]. 

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Fig. 7.24 Enantiomer separation of D,L-phenylalanine on the anti-L-amino acid antibody-type CSP at 1 mL min" and 4 mL min". Separations were carried out at 25 °C using phosphate buffered saline as mobile phase. (Reprinted with permission from [279]).

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