Antibody oligopeptides, synthetic

Mendelson, E., Trempe, J. P. and Carter, B. J. (1986). Identification of the trans-acting Rep proteins of adeno-associated virus by antibodies to a synthetic oligopeptide. J. Virol. 60, 823-832. 

In spite of Its power, ICC is a technique with certain limitations in its specificity (6,7), and in most cases, the technique needs to be supplemented with rigorous specificity testing and independent nonimmunological assay techniques. Neuropeptides, like other peptides and proteins, because of their complex chemical structure, are especially problematic to detect specifically. The antigens used for immunization to obtain neuropeptide antibodies are commonly synthetic oligopeptides coupled to larger carrier proteins. It is known... 

Antibodies were raised in rabbits against synthetic oligopeptides that corresponded to the 15 amino acid residues at the carboxyl termini of the four desaturases of Synechocystis (9, 11) and the oj3 desaturase of A. thaliana. (12). These carboxyl-terminal regions were chosen because they are specific to the respective desaturases. For ImmunoGold labeling, cells of Synechocystis and isolated thylakoid... 

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