Antibody binding energy

The ability of enzymes to preorganize reactants for reaction contributes significantly to their efficacy [21]. The excellent shape complementarity observed in antibody-antigen complexes suggests that antibody binding energy could be exploited for this purpose. Antibody catalysts have been developed for bimolecular Diels-Alder reactions to test this supposition. 

Strain and distortion have also been utilized to achieve large rate accelerations with antibodies. In this strategy, antibody binding energy is used to force a substrate into a destabilizing microenvironment. For example, antibodies are raised against an A-methylated... 

M. M. Mader, P. A. Bartlett, Binding Energy and Catalysis The Implications for Transition-State Analogs and Catalytic Antibodies , Chem. Rev. 1997, 97,1281-1301. 

Freedom of Information Electronic Reading Room (ODER), 15 701-702 Free electron lasers (FELs), 1 720 Free energy, of antigen-antibody binding events, 74 138... 

Furthermore, antibodies should be capable of efficiently catalyze reactions with unfavorable entropies of activation by acting as entropy traps the binding energy of the antibody being used to freeze out the rotational and translational degrees of freedom necessary to form the activated complex. This principle has been applied to the design of antibodies that catalyze both unimolecular and bimolecular reactions (see below). 

Novotny J, Bruccoleri R, Saul F (1989) On the attribution of binding energy in antigen-antibody complexes MCPC 603, D1.3, and HyHEL-5, Biochemistry, 28 4735... 

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