Antibodies polyclonal immunofluorescence

Alternatively, GFP can be visualized using rabbit polyclonal antibody raised against GFP purified directly from A. victoria. This anti-GFP antibody facilitates the detection of native GFP, recombinant GFP, and GFP-fusion proteins both by immunofluorescence and brightfield microscopy, as well as by western blot analysis and immunoprecipitation. Direct anti-GFP conjugates made from a complete serum or from an IgG fraction are available from Invitrogen (http //www.invitrogen.com/ site/us/en/home.html). Additional options for your research offered by Invitrogen include two mouse monoclonal antibodies and a chicken IgY fraction. 

An immunohistochemical examination of PSA using polyclonal antibodies by the peroxidase antiperoxidase (PAP) method and by the technique of biotin-streptavidin-alkaline phosphatase has been successfully carried out (Zaviacic et al., 1994). Immunoelectron microscopy in conjunction with the protein A-gold complex can also be used for localizing PSA in human prostate (Sinha et al., 1987). The procedure for immunofluorescence localization of PSA is given below. 

Fig. 1. Double immunofluorescence detection of cytoplasmic cytokeratin 19 intermediate filaments (CK19) (FITC, green/ filamentous) and the cell-membrane associated connexin 43 (Cx43) gap junction protein (Cy3, red/dot-like) in the stratified squamous epithelium of human cervix. Combination of mouse monoclonal (CK19) and rabbit polyclonal (Cx43) antibodies and distinct fluorochrome-labeled secondary antibodis. Confocal laser scanning microscopy, a projection of five optical layers.

The 30-year history of MAbs has been a rollercoaster ride to success. From hype to depression, and back to hype, is probably the shortest summary of what has happened. MAbs have been rapidly introduced into a number of applications within and outside the medical field (Table 1.2) [52-54]. Analytical in vitro methods such as enzyme-hnked immunosorbent assay (ELISA), radioimmunoassay (RIA), various blotting techniques, flow-cytometry, immunofluorescence, confocal imaging, and im-munohistochemistry are each dependent upon the use of polyclonal or monoclonal antibodies. 

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