Antibodies periodate-oxidized

Figure 10.3 Antibody molecules oxidized with sodium periodate to create aldehyde groups on their polysaccharide chains can be modified with PDPH to produce thiols after reduction of the pyridyl disulfide. Direct labeling of the sulfhydryls with "Tc produces a radioactive complex.

Conjugation of Periodate-Oxidized HRP to Antibodies by Reductive Animation... 

Conjugation of Periodate-Oxidized Antibodies with Amine orHydrazide Derivatives... 

For conjugation to hydrazide-containing proteins, dissolve the periodate-oxidized antibody at a concentration of lOmg/ml in 0.1M sodium phosphate, 0.15M NaCl, pH 6.0-7.2. For conjugation to amine-containing molecules and proteins, dissolve the oxidized antibody at lOmg/ml in 0.2M sodium carbonate, pH 9.6. 

Figure 20.14 Periodate oxidation of HRP creates aldehyde groups on the carbohydrate chains of the enzyme. Reaction with a Fab fragment then may be done using reductive amination to produce a lower-molecular-weight complex than would be obtained using intact IgG antibodies.

Figure 21.15 A periodate-oxidized dextran polymer may be reacted with both an antibody and an intact toxin component using reductive amination to form a multivalent immunotoxin complex.

Woodward, M.P., Young, W.W., and Bloodgood, R.A. (1985) Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation./. Immunol. Meth. 78, 143-153. 

Many types of mono-, bi-, and multifunctional coupling reagents are available for labeling antibodies or antigens with an enzyme. Glutaraldehyde, carbodiimide, N-succinimidyl-3-(2-pyridyldithio)propionate, and periodate oxidation of carbohydrate moieties to form active dialdehydes are several commonly used approaches in the preparation of enzyme conjugates (104-106). 

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