Antibodies hydrazide derivatives

Conjugation of Periodate-Oxidized Antibodies with Amine or Hydrazide Derivatives... 

Prepare the labeling reagent stock solution. Biotin, biocytin, and desthiobiotin hydrazide derivatives are typically used at a labeling reagent antibody MR of 20-25. Biotin and desthiobiotin hydrazide stock solutions should be prepared at 10-20 mg/mL in DMSO or DMF while biocytin hydrazide can be dissolved in the reaction... 

A synthesis of MTX y-hydrazide (VIII.224) was described in 1981 by Rosowsky et al. [299], Condensation of MeAPA with y-methyl a-/-butyl L-glu-tamate with the aid of diethyl phosphorocyanidate afforded the diester (VIII.225) (76% yield), which was then converted sequentially to (VIII.226) (60% yield) on treatment with hydrazine in MeOH solution at 4 °C for 3 days and to (VIII.224) (45% yield) on hydrolysis with 1 M HCl at 50 °C for 1 h. Compound (VIII.224) has gained attention recently because of the opportunity it affords to couple MTX to monoclonal antitumour antibodies through the formation of semicarbazide bonds to periodate-oxidized sugars [331]. The y-hydrazide derivative (VIII.227) of AMT has also been cited briefly [332], although details of its synthesis have not yet been disclosed. 

G. Fleminger, E. Hadas, T. Wolf, and B. Soloman, Oriented immobilization of periodate-oxidized monoclonal antibodies on amino and hydrazide derivatives of Eupergit C, Appl. Biochem. Biotech., 23 (1990) 123-137. 

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