Antianxiety and Antipsychotic Drugs

Diazepam, three metabolites (A -desmethyldiazepam, temazepam, oxazepam) and clonazepam [IS] were isolated from plasma or urine and separated on a base deactivated C g column (2 = 232nm). A 50/10/40 methanol/acetonitrile/water (50 mM KH2PO4 pH 3.5) mobile phase generated baseline resolution in 12 min [552]. Calibration standards ran from 10 to 2000ng/mL with detection limits of lOng/mL reported. Similarly, diazepam and three metabolites (nordiazepam, oxazepam, temazepam) were baseline resolved in 12min on a Cjg column (A = 257 nm) using a 60/35/5 methanol/water/acetonitrile mobile phase [553]. 

The degradation of lorazepam in acidic solution was ftrllowed on a base deactivated Cg colunm (A = 230nm). A 35/20/45 methanol/acetonitrile/water (0.1 M ammonium acetate with 5mM KH2PO4 to pH 6 with acetic acid) mobile phase resolved lorazepam fiom 2-amino-2,5-dichlorobenzophenone and 6-chloro-4-(2-chlorophenyl)-2-quinazolinecarboxaldehyde in 17 min. A linear range of 0.5-1.8 pg/mL and detection limits of 0.2 pg/mL were reported [556]. 

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