Anti-lactose antibodies preparation

The homogeneity and molecular size of the purified antibody preparations were determined by ultracentrifiigation in a Spinco Model E centrifuge. The results of this analysis on anti-lactose antibodies are illustrated in the photographs of the ultracentrifuge patterns shown in Fig. (1). The... 

Gel electrophoresis yielded a broad band and indicated several protein components, Fig. (15B). It was necessary to establish whether the preparation contained several types of antibodies or only anti-lactose antibodies. The preparation was subjected to electrofocusing [39], Duplicate gels were made by electrofocusing. One gel was stained for protein and showed the presence of multiproteins, Fig. (15C). The unstained gel was examined by the coupled method and yielded a precipitin which was opposite each band in the stained gel, Fig. (15D-F). These results showed that the purified antibody preparation consisted of 13 protein species each with antibody activity directed at lactose-containing antigen. 

That both components were antibodies was established by agar diffusion tests with these preparations and solutions of the glycan performed in the usual manner. The results of the agar diffusion tests are shown in Figure 3, right plate. The two preparations of antibodies have been designated as anti-galactose (anti-gal) antibodies and anti-lactose (anti-lac) antibodies and some of the properties of the two sets of antibodies are described in a later section. 

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