Anomalies and Corrections in Collecting Electrophoresis

Broad fraction Diffusion due to paper Machine direction vertical 

No separation Evaporation flow in the horizontal sense Flooding of paper from the electrodes Good dissipation of heat (thin wall and cooling system) reduction of heat (lower n, thinner paper) Regulation of electrode flow or cascade electrodes 

Malformation Irregular buffer flow Through folded sub- Machine direction vertical 

Initial lack of separation Modification, due to the sample, of pH and n of background buffer Dialysis of sample or slower feeding 

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