Anisotropies with Multiphoton Excitation

ANISOTROPIES WITH MULTIPHOTON EXCITATION Advanced Topic  

As shown in Eq. [10.17], the anisotropy is essentially the average value of cos 0. The fundamental anisotropy value of 0.4 for one-photon excitation is a consequence of cos 0 photoselecdon (Eq. [10.1 ]). For two-photon excitation, die fluorophore interacts simultaneously with two photons, and each interaction is proportional to cos 0. Hence, die photoselecdon function becomes  

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T0.13. ANISOTROPIES WITH MULTIPHOTON EXCITATION Advanced Topic ... 

The technique of transient grating spectroscopy has been reviewed, with particular emphasis on its application to monitoring non-radiative deactivation. A unified theory of time-resolved fluorescence anisotropy and Stokes shift spectroscopy has appeared. A separate review has considered the chemical and photophysical events occurring from upper excited states as accessed by multiphoton absorption techniques. ... 

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