Anisaldehyde reagent

Euphane triterpenes Schinus molle SiOj T - AcOEt - AcOH Anisaldehyde reagent Qualitative identification 52 n 3"... 

Figure 16.4 displays the separation of the resins of B. carterii and B. serrata, their hydrodistillates, and three commercially available olibanum essential oils on a Merck LiChrospher plate in the mobile phase heptane-diethylether-formic acid (7 + 3 + 0.3 v/v/v) without chamber saturation after derivatization with anisaldehyde reagent. 

The TLC screening of the sihca gel fractions of B. carterii revealed in fraction 1, after derivatization with anisaldehyde reagent, a single violet zone (Rf 0.85). The purification steps of this zone are illustrated in Figure 16.5. The zones of the following fractions 2 and 3 were not separated from each other. Their separation and purification steps are shown in Figure 16.6, whereas the appropriate steps of fraction 4 are demonstrated in Figure 16.7. 

After development to a distance of 7 cm with cyclohexane-diethyl ether (80 + 20 v/v) as the mobile phase and derivatizing with anisaldehyde reagent (Figure 16.10),... 

Most of the reactions described in the following chapters were monitored by Thin Layer Chromatography (TLC) using plastic TLC plates coated with a thin layer of Merck 60 F254 silica gel. The products were detected by using an ultraviolet lamp or the TLC plates were treated with p-anisaldehyde reagent, prepared as explained below, and then heated to 120 °C to stain the spots. After visualization and measurement, the Rf values were recorded. 

Thin-layer Chromatography. System TK—Rf 82. (p-Anisaldehyde reagent, blue perchloric acid solution, followed by examination under ultraviolet light, red fluorescence.)... 

Ammonium Polysulphide Solution dissolve a sufficient quantity of precipitated sulphur in ammonium sulphide solution to produce a deep orange solution Ammonium Sulphide Solution saturate 120 ml of 5M ammonia with washed hydrogen sulphide and add 80 ml of 5M ammonia It should be recently prepared p-Anisaldehyde Reagent dissolve 0 5 ml of p-anisaldehyde in 50 ml of acetic acid and 1 ml of hydrochloric acid... 

Heat the plate and then dip it into the anisaldehyde reagent as before. Record which tubes have sample in them. 

Kritchewski et al. [104] recommend an anisaldehyde reagent (No. 11) which yields blue to pink spots (1 (jig). 

C. After spraying the plates with anisaldehyde reagent [95% ethanol-conc. phosphoric acid-anisaldehyde (9 1 1)] and heating to I00°C for 5-10 min in an oven, the conversion product of niddamycin was characterized by a rather unique purple-red color... 

Visible as reddish spot after spraying with p-anisaldehyde reagent and heating to IIO C for S-10 min Rr... 

FIGURE 4.4 Chromatograms showing chiral resolution of racemic compounds on (a) Microcrystalline cellulose frw(3,5-dimethylphenyl)carbamate of cellulose in the 1 1 (w/w) ratio as stationary phase. Eluent n-hexane/propan-2-ol (80 20, v/v). Visualization anisaldehyde reagent. Migration distance = 6 cm at room temperature. Enantiomers and racemic propranolol [36]. (b) Microcrystalline cellulose tris(2,3-dimethylphenyl)carbamate of cellulose in the 1 1 (w/w) ratio as stationary phase. Eluent n-hexane/propan-2-ol (90 10, w/w). Enantiomers and racemic bupranolol [36]. (c) Amino-propyl sUanized sihca gel (3 g) coated with rnj(cyclohexyl)carbamates of amylose (0.75 g) as stationary phase. Eluent n-hexane/propan-2-ol (90 10, v/v). Racemates (a) 2,2,2-trifluoro-(9-antryl)ethanol, (b) Troger s base, and (c) benzoin ethyl ether. Visualization UV (254 nm). Migration distance = 6.2 cm at room temperature [37]. 

B 45(-) 54 (+) 1.43 — Detection anisaldehyde reagent (blue or purple-blue spots)... 

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