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Anion exchange chromatography, native separation

The application of LC online with ESTMS is especially attractive for the speciation analysis of Cd and Zn complexes. Metallated complexes such as MTs may suffer from several artifacts in their chromatographic separation due to the presence of those metals. For instance, the presence of other metals may modify the retention of the complex Cd-Zn-MTs on a RP column. As an alternative, ESI-MS with pH control can help in the analysis of metals in native and reconstituted metalloproteins, in order to exactly elucidate which biocompoimd binds Cd, how many atoms of Cd per bioHgand molecule are boimd, and what is the combination with other metals (e.g., Zn and Cu) in such molecules. In this line, samples of rabbit liver and horse kidney MTs (previously isolated by SEC and anionic-exchange chromatography) were analyzed imder pH selected conditions. In moderately acidic (pH 4.0) and neutral (pH 6-7) media, the complexes of MTs with Cd and Zn retain... [Pg.330]

See other pages where Anion exchange chromatography, native separation is mentioned: [Pg.18]    [Pg.43]    [Pg.1492]    [Pg.55]    [Pg.397]    [Pg.89]    [Pg.710]    [Pg.841]    [Pg.1146]    [Pg.272]    [Pg.143]    [Pg.261]    [Pg.312]    [Pg.53]    [Pg.842]   
See also in sourсe #XX -- [ Pg.23 ]



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