Aniline hydrolase

Species Aniline hydrolase d-Benzphetamine demethylase 7-Ethoxycoumarin deethylase... 

Interestingly, hydrolase-type enzymes could also be employed for enantioselective hydrations79. Yields and enantiomeric excess values obtained in the addition of water to 2-(trifluo-romethyl)propenoic acid (7) are summarized. Unfortunately, the absolute configuration of the product 8 was not determined, the enantiomeric excess values stated rest on 19F-NMR analysis of the Mosher ester. Whereas ethanol and phenol did not react under these conditions, the /V-nucleophiles diethylamine and aniline, and also thiophenol, could be added to 7 in moderate to good yields and enantiomeric excess values. Finally, the bifunctional aniline derivatives 9 afforded the lactams 10 in 52-90% yield and 25-67% ee. Again, the absolute configurations of the products were not determined. 

Apart from natural materials, oxidoreducates have been used to modify synthetic polymers. For example, using peroxidase, poly(4-hydroxystyrene) has been functionalized with aniline while poly(p-phenylene-2,6-benzobisthiazole) has been rendered more hydrophilic [22, 23]. Other authors have demonstrated that phenolics can be covalently bound to amino-functionalized polymers by using laccase resulting in increased fire resistance [13]A large number of scientific reports are available on enzymatic functionalization of poly(alkyleneterephthalate)s. Polyester fibers account for 73% of all synthetic fibers on the market with an annual production of approx. 27 million tons [24]. Similarly, polyamides and polyacrylonitriles have significant market shares. In contrast to natural polymers discussed above, hydrolases have shown higher potential for modification of these synthetic materials than oxidoreducates. 

Invertase, fi-D-fructofuranosidase (EC 3.2.1.26) a saccharide-cleaving hydrolase from yeast, fungi and higher plants. Yeast I. is a dimeric ycoprotein (Af, 270,000), Neurospora invertase a tetrameric glycoprotein (M, 210,000, Af, of the subunit, 51,000). Aniline (fungal I.), pyridoxal (potato I.) and zinc ions (all I.) are inhibitors of I. 

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